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Applications

The application notes and videos available here help to illustrate how Isco instruments can help solve your monitoring, testing, and analysis problems.

Our application notes are stored in Adobe Acrobat PDF format, and require an Acrobat reader (available here).

Liquid Chromatography
Application Notes
AN01 Peptide Separations Using Reversed Phase RediSep Columns (PDF: 65K, Aug 2003)
AN02 C18 Reversed Phase RediSep Columns Use, Cleaning, and Storage (PDF: 56K, Jan 2007)
AN02 C18 Reversed Phase RediSep Columns Use Cleaning and Storage (PDF: 87K, Jun 2009)
AN03 Optimal Flow Rates for RediSep Normal Phase Columns (PDF: 73K, Aug 2003)
AN04 Purification by Liquid Chromatography (PDF: 72K, Aug 2003)
AN05 Separation of Lipids by Normal Phase Flash Chromatography (PDF: 56K, Aug 2003)
AN06 Improved Productivity with Isco RediSep Flash Columns (PDF: 126K, Aug 2003)
AN07 Operating RediSep Flash Columns Using Pressurized Solvent Reservoir Systems (PDF: 508K, Oct 2004)
AN08 Introduction to Functionalized Silica Gel and Alumina (PDF: 455K, Aug 2003)
AN09 Locating Fractions in Collection Tubes using Rf to CV Conversion (PDF: 540K, Oct 2003)
AN12 RediSep Normal Phase Column Stacking (PDF: 513K, Dec 2004)
AN13 RediSep Column Stacking Technique (PDF: 493K, Jun 2005)
AN15 Dry samples improve resolution in normal phase flash chromatography (PDF: 86K, Jul 2006)
AN16 Improved Productivity With RediSep Columns (PDF: 130K, Apr 2004)
AN19 Using RediSep Flash Columns on FlashMaster Chromatography Systems (PDF: 461K, Oct 2004)
AN20 Acetone as an Alternative to Ethyl Acetate (PDF: 133K, Apr 2008)
AN21 CombiFlash Rf System Verification Using Test Sample NPHE (PDF: 76K, Jun 2010)
AN22 Expanded Compound Wavelength Detection with UV-Vis (PDF: 61K, Nov 2008)
AN23 Self-pack Column Options for the CombiFlash Rf (PDF: 144K, Aug 2011)
AN24 TLC Isocratic and Gradient (PDF: 478K, May 2004)
AN25 Purification of Natural Products Case Study 1 Scutellaria baicalensis (PDF: 130K, Mar 2007)
AN26 Isolating Trace Quantities of Product (PDF: 451K, Jul 2005)
AN27 Rapid Purification of Tocopherols (PDF: 220K, Feb 2009)
AN28 Solvent Changing on CombiFlash Systems (PDF: 149K, Nov 2010)
AN29 Overview of Silica Column Sample Loading Techniques (PDF: 656K, Dec 2010)
AN31 RediSep Amine Column - Purification of high pKa Organic Compounds Case Study 1 (PDF: 139K, Nov 2005)
AN35 RediSep Basic Alumina Column - Purification of High pKa Organic Compounds Case Study 1 (PDF: 91K, Oct 2010)
AN39 RediSep SCX Column - Purification of High pKa Organic Compounds Case Study 1 (PDF: 470K, Jun 2005)
AN40 Purification of Alkaloids with RediSep Rf SCX Columns (PDF: 245K, Oct 2011)
AN44 RediSep SAX Column - Purification of acidic compounds with multipurpose scavenger column Case Study 1 (PDF: 489K, Jul 2005)
AN49 Improvements in RP MPLC as Alternative to Prep HPLC (PDF: 207K, Nov 2008)
AN50 RediSep C-18 Column - Purification of halogenated heterocyclic compounds (PDF: 469K, Jun 2005)
AN51 RediSep C-18 Column - Purification of peptides (PDF: 452K, May 2005)
AN52 RediSep C-18 Column - Purification of carbohydrates (PDF: 464K, May 2005)
AN53 RediSep C-18 Column - Purification of carboxylic acids (PDF: 464K, May 2005)
AN54 RediSep C-18 Column - Purification of primary amines (PDF: 465K, May 2005)
AN55 RediSep C-18 Column - Purification of low-solubility polar heterocycles (PDF: 466K, May 2005)
AN56 RediSep C-18 Column - Purification of ionic compounds (PDF: 466K, May 2005)
AN57 Acetonitrile Methanol Substitution in C18 Reverse Phase (PDF: 175K, Nov 2009)
AN58 Non-aqueous Reverse Phase with RediSep Rf Gold C18 (PDF: 123K, Nov 2011)
AN59 Purine Purification Strategies (PDF: 1335K, Jan 2011)
AN60 RediSep Neutral Alumina Column - Purification of High pKa Organic Compounds Case Study 1 (PDF: 468K, Jun 2005)
AN65 RediSep Amine Columns Use Cleaning and Storage (PDF: 125K, Jun 2009)
AN70 Higher Resolution Results with RediSep Rf Gold Silica Columns (PDF: 167K, Mar 2009)
AN71 RediSepRf Gold Columns for Purification of Minor Compounds (PDF: 122K, Mar 2009)
AN72 Improved Productivity and Savings with RediSep Rf Gold Columns (PDF: 142K, Mar 2009)
AN73 RediSep Rf Gold Columns on Companion XL (PDF: 238K, Mar 2009)
AN74 Use of RediSep Rf Gold C18 Columns at High pH (PDF: 201K, Aug 2009)
AN75 Strategies to Purify Carbohydrate Based Compounds (PDF: 468K, Aug 2009)
AN76 RediSep Rf Gold C18A for Highly Aqueous Mobile Phases (PDF: 307K, Feb 2011)
AN77 Wide Polarity Range Chromatography (PDF: 324K, Jun 2010)
AN78 HILIC Purification Strategies for Flash Chromatography (PDF: 265K, Oct 2010)
AN80 Evaporative Light Scattering Detectors (PDF: 238K, Jun 2010)
AN81 CombiFlash All-wavelength Collection (PDF: 546K, Jun 2010)
AN82 Comparison of Solid Load Cartridges and Reservoir Type Columns (PDF: 440K, Dec 2010)
AN83 Multi-step Synthesis and Purification (PDF: 438K, May 2011)
AN85 Purification Strategies for Flavones and Related Compounds (PDF: 159K, Nov 2011)
Article Reprints
Networked Purification System Increases Productivity (PDF: 783K, Nov 2006)
Preparative Parallel Protein Purification (PDF: 205K, Mar 2005)
Poster and Paper Reprints
AdvancedTopics RediSepSpecialtyMedia (PDF: 1565K, Apr 2007)
Advanced Topics RediSep Specialty Media (PDF: 1565K, Apr 2007)
Advances In Medium Pressure Liquid Chromatography (PDF: 302K, Mar 2009)
Alkaloid Purification Strategies Using Flash Chromatography (PDF: 950K, Sep 2011)
All Wavelength Detection MPLC for Natural Products (PDF: 658K, Jul 2009)
C-18 Chromatography- From Analytical Scale to Preparative Scale (PDF: 355K, Nov 2006)
Diol Columns-Pretend They're Normal Phase (PDF: 657K, Apr 2010)
Facile Enrichment of Tocopherols (PDF: 1107K, Mar 2009)
Facile Isolation of Carotenoid Antioxidants (PDF: 855K, Apr 2010)
Flash Chromatography of Hi pKa Compunds with Specialty RediSep Columns (PDF: 336K, Aug 2004)
Flash Purification of Polar Organic Compounds Using C-18 RediSep (PDF: 138K, May 2005)
Isolation and Purification of Alkaloids with SCX Columns (PDF: 644K, Aug 2011)
Method Development Strategies for Amine Columns (PDF: 505K, Apr 2010)
Minimize Purification Time with High Resolution Flash Chromatography (PDF: 208K, Aug 2004)
Minimizing Protein Purification Time with Parallel Purification (PDF: 824K, Jun 2005)
Optimized Flash Chromatography- From TLC to large scale in 3 steps (PDF: 346K, May 2006)
Purification of Carbohydrates by MPLC (PDF: 886K, Aug 2009)
Sample Loading techniques for Large Scale Flash Chromatography (PDF: 810K, Aug 2010)
Spherical Silica Increases Loading Capacity (PDF: 1428K, Aug 2009)
Strategies for the Flash Purification of Highly Polar Compounds (PDF: 708K, Jan 2012)
Presentations
OPopS Optimer One-pot Synthesis (PDF: 193K, Apr 2005)
Tactics Addressing Challenging Flash Chromatography Purifications (PDF: 589K, Apr 2005)
Technical Notes
TN01 Flowcell Care and Cleaning for CombiFlash Companion (PDF: 463K, Jul 2005)
TN02 Networked Purification System Increases Productivity for Medicinal Chemists (PDF: 968K, Apr 2004)
TN03 Companion Direct Communication Issues (PDF: 457K, May 2004)
TN04 Companion Network Communication Issues (PDF: 460K, Jun 2004)
TN05 Companion iPAQ Communication Issues (PDF: 452K, May 2004)
TN06 Controlling the Companion without Wires (PDF: 455K, May 2004)
TN07 Companion Periodic Maintenance (PDF: 174K, Jan 2006)
TN09 Companion Solvent Change between Normal and Reversed Phases (PDF: 128K, Jun 2006)
TN10 Adjustable Solid Load Cartridge Cap (PDF: 134K, Jan 2007)
TN11 Adjustable Rf Solid Load Cartridge Cap (PDF: 256K, Sep 2007)
TN12 Adjustable Solid Load Cartridge Cap (PDF: 255K, Jun 2009)
TN13 Guide to Cartridges and Caps (PDF: 119K, Apr 2009)
TN19 Fraction Tube Filling Variability on Rf200 Systems (PDF: 51K, Jan 2011)
TN20 Solid Load Cartridge Bypass (PDF: 146K, Aug 2011)
High-Precision, High-Pressure Syringe Pumps
Application Notes
Aqueous-PhaseConversion Biomass (PDF: 81K, Feb 2012)
Aqueous Solubility at High Temperature (PDF: 333K, Jan 2012)
Carbon Sequestration (PDF: 82K, Sep 2008)
Catalytic Hydrogenation (PDF: 136K, Aug 2008)
CO2 Injection (PDF: 69K, Jun 2008)
Core Analysis (PDF: 86K, Feb 2008)
Enhanced Oil Recovery (PDF: 111K, Nov 2007)
Exploring New Energy Concepts (PDF: 109K, Jul 2008)
Extrusion Foaming (PDF: 616K, Dec 2008)
Foam Injection Molding (PDF: 75K, Oct 2008)
Gels for Gas EOR Flooding (PDF: 92K, May 2007)
HPLC System Configuration (PDF: 135K, Apr 2008)
Measurement of Binary Diffusion Coefficients as Function of Temperature (PDF: 193K, Dec 2011)
Methane Hydrate Studies (PDF: 122K, Mar 2011)
Microreactor Systems (PDF: 134K, Jun 2008)
Oil Sands Recovery (PDF: 85K, Jun 2008)
PBA Solubility Measurement (PDF: 224K, Dec 2008)
Plastic Foaming Simulation (PDF: 169K, Jan 2009)
Processing of Aqueous Biomass (PDF: 74K, Feb 2012)
Pumping Biomass Isco HVPumps (PDF: 337K, Jun 2009)
Reactant Feed (PDF: 94K, Aug 2010)
Reactive Spray Deposition (PDF: 41K, Dec 2007)
SeismicStudies CO2Sequestration EOR (PDF: 310K, Dec 2008)
Semi-Continuous Pretreatment Lignocellulosic Biomass (PDF: 208K, Jan 2012)
SFE Preparation for Radiocarbon Dating (PDF: 134K, Jan 2012)
SlurryMixer Particle Suspension (PDF: 197K, Feb 2010)
SupercriticalCO2 (PDF: 92K, Aug 2007)
Supercritical Fluids in Reaction Engineering (PDF: 154K, Aug 2007)
Ultra HPLC System Configuration (PDF: 120K, Mar 2008)
X-Ray Computed Microtomography (PDF: 173K, Jan 2008)
Xanthan-HPAM Rheology Comparison (PDF: 101K, Jan 2008)
Software Utilities
Labview Pump Driver demo (ZIP: 1598K, Oct 2005)
SFSolver2000 (EXE: 45K, Mar 2003)
Universal D-series Pump driver (ZIP: 106K, Oct 2005)
Technical Bulletins
TB01 Dual Pump Systems (PDF: 729K, Sep 2010)
TB02 Receive Mode (PDF: 471K, Feb 2009)
TB03 Syringe Pump Analog Options (PDF: 483K, Jan 2010)
TB04 Salt Solutions & Brines (PDF: 284K, Jan 2009)
TB05 Field Verification (PDF: 585K, Mar 2010)
TB06 LabView Toolkit (PDF: 613K, Mar 2011)
TB07 Temperature Control Jacket (PDF: 241K, May 2011)
TB08 Pumping CO2 Setup&Notes (PDF: 316K, Apr 2011)
TB09 Flash Memory Upgrade (PDF: 344K, Jun 2010)
TB10 Low Flow Operation (PDF: 295K, Nov 2010)
TB11 Dispense Mode (PDF: 220K, Jun 2010)
TB12 Non-Isco Transducer Installation (PDF: 482K, Jun 2010)
TB13 Dimensions Connections (PDF: 430K, Sep 2010)
TB14 SingleValve Packages (PDF: 339K, Oct 2010)
TB15 Metering Pumps (PDF: 317K, Oct 2010)
TB16 Pumping Hazardous Substances (PDF: 174K, Dec 2010)
TB17 High Temperature High Accuracy (PDF: 197K, Dec 2010)
TB18 Pumping Highly Viscous Fluids (PDF: 369K, Mar 2011)
TB19 Universal Driver (PDF: 118K, Mar 2011)
TB20 Modifier Addition (PDF: 584K, Apr 2011)
TB21 Cylinder&System Flushing (PDF: 229K, May 2011)
TB22 SyringePump Care&Maintenance (PDF: 1074K, Jun 2011)
TB23 Diagnostics&Troubleshooting (PDF: 388K, Oct 2011)
Videos and Animations
Dual Pump Operation Example (SWF: 20K, Mar 2004)
Pump Overview (SWF: 106K, Sep 2010)
Single Pump Operation Example (SWF: 24K, Jun 2004)
Wash Port Operation Example (SWF: 32K, Nov 2004)
Webinars
Pumping High Viscosity Fluids 13min (SWF: 6466K, May 2011)
White Papers
Unconventional Natural Gas Recovery (PDF: 1835K, Mar 2011)
Laboratory Autosamplers
Density Gradient Fractionation
DGF11 Comparison of Methods to Retrieve Samples from Density Gradient Solutions (PDF: 465K, Aug 2003)
DGF12 Effects of Flow Cells on Sample Resolution in Density Gradient Fractionation (PDF: 464K, Aug 2003)
Automatic Water Samplers
Isco Stormwater Monitoring Guide (PDF: 549K, Jul 2004)
Nicad vs LeadAcidBatteries TechBulletin (PDF: 83K, Jun 2000)
Application Notes and Articles
Active Stormwater Runoff Monitoring (PDF: 464K, May 2010)
Isco Samplers for Research (PDF: 94K, Jan 2004)
Radionuclide Sampling using SPE disks (PDF: 820K, Jun 2003)
Remote Runoff Monitoring (PDF: 195K, Jan 2006)
Selection of Automatic Samplers Article (PDF: 190K, Jan 2003)
SW846 Trace Metal Sampling using ProPak Disposables (PDF: 92K, Feb 2007)
Case Studies
4700Sampler Industrial Park Wastewater Monitoring Germany (PDF: 385K, Sep 2009)
6712Sampler Remote Industrial Monitoring Spain (PDF: 228K, Mar 2009)
Simultaneous Watershed Sampling Springfield (PDF: 416K, Apr 2010)
Wastewater Sampling at High Ambient Temp (PDF: 187K, May 2010)
Presentations
Stormwater Monitoring Webinar Oct 2010 (PDF: 4911K, Oct 2010)
Webinar
Advanced Stormwater Monitoring - 20Mb Open in Web Browser (SWF: 20735K, Aug 2011)
U.S. Industrial Pretreatment Monitoring (WMV: 26838K, Feb 2012)
Open Channel Flow Measurement
ADFM Installation Data
ADFM 4-20mA Analog Output Module Diagram (PDF: 17K, May 2006)
ADFM Environmental Enclosure Console (PDF: 498K, May 2006)
ADFM Sensor Mounting Bands for Circular Pipes (PDF: 143K, May 2006)
ADFM Typical Installation Drawing- External Power (PDF: 515K, May 2006)
Application Notes
4501 PumpStationPerformanceMonitor (PDF: 216K, Jun 2009)
Active Stormwater Runoff Monitoring (PDF: 464K, May 2010)
HotTapInsertion VelocityProfiling in VariedConditions (PDF: 218K, Feb 2010)
RemoteWaterMonitoring 2105G Brazil (PDF: 368K, Aug 2011)
Case Studies
2150AV I&I AvedoreWWTP Denmark (PDF: 228K, May 2009)
ADFMPro20 WWTP CzechRepublic (PDF: 208K, Jul 2010)
Billing Application PassaicValley NJ (PDF: 333K, Apr 2010)
Cooling Water Monitoring-Austria (PDF: 318K, Oct 2011)
HotTap Article WWTP PollutionEngineering (PDF: 925K, Sep 2007)
HRSD Large Pump Station HTI Monitoring (PDF: 612K, Dec 2009)
HTI Closed-Pipe Monitoring Indianapolis (PDF: 432K, Sep 2010)
HTI PumpStation Denmark (PDF: 253K, Jan 2011)
I&I Solutions FallsCityNE (PDF: 242K, Dec 2009)
Large-Pipe I&I TwinCities-MN (PDF: 257K, Apr 2010)
Large Inlet Profiling Biberach Germany (PDF: 321K, Nov 2010)
RemoteMonitoring AirportRunoff Ireland (PDF: 248K, Mar 2011)
Remote CSO 2105G UK (PDF: 385K, Aug 2011)
Stormwater Clean-Up NuRF Florida (PDF: 253K, Mar 2010)
WWTP AerationBasin Sweden (PDF: 212K, Jul 2010)
Papers and Article Reprints
Advanced Technology Flow Logging Under ATEX IET reprint (PDF: 913K, Jun 2005)
Avoiding the Pitfalls of Dynamic Hydraulic Conditions with Real-time Data (PDF: 325K, May 2006)
Flow Measurement for Collection Systems April05 PollutionEngineering (PDF: 539K, Jun 2005)
HotTap Article Aug07 PollutionEngineering (PDF: 925K, Sep 2007)
Industrial wastewater monitoring without manhole entry (PDF: 52K, Dec 1999)
ITRC Report 01-004 Open Channel Doppler Flow Meter (PDF: 350K, May 2006)
Measuring Sacramento River Diversions with ADFM Technology in the Glenn-Colusa Irrigation District (PDF: 996K, Sep 2007)
OpenChannelDoppler AV Article (PDF: 18K, Dec 1999)
OpenChannelFlowMeters Article (PDF: 34K, Dec 1999)
Pump Station Monitor Helps Village of Kohler (PDF: 158K, Dec 2004)
USCID 1999 Conference- Velocity Profiling Technology for Accurate Water Management (PDF: 106K, May 2006)
USCID 2002 Conference- Novel Flow Sensor Saves USBR 900K USD (PDF: 1395K, May 2006)
WEFTEC 1997 Conference- Velocity Profiling for Wastewater Collection and Treatment (PDF: 663K, May 2006)
Test Reports
ADFM Accuracy Tests Summary (PDF: 163K, May 2006)
Bureau of Reclamation Report- ADFM Lab and Field Demonstrations (PDF: 289K, May 2006)
FM Global IS Certificate of Compliance for ADFM (PDF: 134K, May 2006)
Onondaga County M5 Dye Study (PDF: 30K, May 2006)
USU Water Research Laboratory ADFM Accuracy Test Results (PDF: 27K, May 2006)

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