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Applications for Liquid Chromatography

The application notes and videos available here help to illustrate how Isco instruments can help solve your monitoring, testing, and analysis problems.

Our application notes are stored in Adobe Acrobat PDF format, and require an Acrobat reader (available here).

Application Notes
AN01 Peptide Separations Using Reversed Phase RediSep Columns (PDF: 65K, Aug 2003)
AN02 C18 Reversed Phase RediSep Columns Use Cleaning and Storage (PDF: 87K, Jun 2009)
AN03 Optimal Flow Rates for RediSep Normal Phase Columns (PDF: 73K, Aug 2003)
AN04 Purification by Liquid Chromatography (PDF: 72K, Aug 2003)
AN05 Separation of Lipids by Normal Phase Flash Chromatography (PDF: 56K, Aug 2003)
AN06 Improved Productivity with Isco RediSep Flash Columns (PDF: 126K, Aug 2003)
AN07 Operating RediSep Flash Columns Using Pressurized Solvent Reservoir Systems (PDF: 508K, Oct 2004)
AN08 Introduction to Functionalized Silica Gel and Alumina (PDF: 455K, Aug 2003)
AN09 Locating Fractions in Collection Tubes using Rf to CV Conversion (PDF: 540K, Oct 2003)
AN10 CombiFlash System Verification Using Test Sample A (PDF: 74K, Dec 2007)
AN11 CombiFlash Rf System Verification Using Test Sample NPHE (PDF: 83K, Oct 2007)
AN12 RediSep Normal Phase Column Stacking (PDF: 513K, Dec 2004)
AN13 RediSep Column Stacking Technique (PDF: 493K, Jun 2005)
AN15 Dry samples improve resolution in normal phase flash chromatography (PDF: 86K, Jul 2006)
AN16 Improved Productivity With RediSep Columns (PDF: 130K, Apr 2004)
AN19 Using RediSep Flash Columns on FlashMaster Chromatography Systems (PDF: 461K, Oct 2004)
AN20 Acetone as an Alternative to Ethyl Acetate (PDF: 133K, Apr 2008)
AN21 CombiFlash Rf System Verification Using Test Sample NPHE (PDF: 76K, Jun 2010)
AN22 Expanded Compound Wavelength Detection with UV-Vis (PDF: 61K, Nov 2008)
AN23 Self-pack Column Options for the CombiFlash Rf (PDF: 144K, Aug 2010)
AN24 TLC Isocratic and Gradient (PDF: 478K, May 2004)
AN25 Purification of Natural Products Case Study 1 Scutellaria baicalensis (PDF: 130K, Mar 2007)
AN26 Isolating Trace Quantities of Product (PDF: 451K, Jul 2005)
AN27 Rapid Purification of Tocopherols (PDF: 220K, Feb 2009)
AN31 RediSep Amine Column - Purification of high pKa Organic Compounds Case Study 1 (PDF: 139K, Nov 2005)
AN35 RediSep Basic Alumina Column - Purification of High pKa Organic Compounds Case Study 1 (PDF: 476K, Jun 2005)
AN39 RediSep SCX Column - Purification of High pKa Organic Compounds Case Study 1 (PDF: 470K, Jun 2005)
AN44 RediSep SAX Column - Purification of acidic compounds with multipurpose scavenger column Case Study 1 (PDF: 489K, Jul 2005)
AN49 Improvements in RP MPLC as Alternative to Prep HPLC (PDF: 207K, Nov 2008)
AN50 RediSep C-18 Column - Purification of halogenated heterocyclic compounds (PDF: 469K, Jun 2005)
AN51 RediSep C-18 Column - Purification of peptides (PDF: 452K, May 2005)
AN52 RediSep C-18 Column - Purification of carbohydrates (PDF: 464K, May 2005)
AN53 RediSep C-18 Column - Purification of carboxylic acids (PDF: 464K, May 2005)
AN54 RediSep C-18 Column - Purification of primary amines (PDF: 465K, May 2005)
AN55 RediSep C-18 Column - Purification of low-solubility polar heterocycles (PDF: 466K, May 2005)
AN56 RediSep C-18 Column - Purification of ionic compounds (PDF: 466K, May 2005)
AN57 Acetonitrile Methanol Substitution in C18 Reverse Phase (PDF: 175K, Nov 2009)
AN58 Non-aqueous Reverse Phase with RediSep Rf Gold C18 (PDF: 124K, Jun 2010)
AN60 RediSep Neutral Alumina Column - Purification of High pKa Organic Compounds Case Study 1 (PDF: 468K, Jun 2005)
AN65 RediSep Amine Columns Use Cleaning and Storage (PDF: 125K, Jun 2009)
AN70 Higher Resolution Results with RediSep Rf Gold Silica Columns (PDF: 167K, Mar 2009)
AN71 RediSepRf Gold Columns for Purification of Minor Compounds (PDF: 122K, Mar 2009)
AN72 Improved Productivity and Savings with RediSep Rf Gold Columns (PDF: 142K, Mar 2009)
AN73 RediSep Rf Gold Columns on Companion XL (PDF: 238K, Mar 2009)
AN74 Use of RediSep Rf Gold C18 Columns at High pH (PDF: 201K, Aug 2009)
AN75 Strategies to Purify Carbohydrate Based Compounds (PDF: 468K, Aug 2009)
AN76 RediSep Rf Gold C18A for Highly Aqueous Mobile Phases (PDF: 296K, Jun 2010)
AN77 Wide Polarity Range Chromatography (PDF: 324K, Jun 2010)
AN80 Evaporative Light Scattering Detectors (PDF: 238K, Jun 2010)
AN81 CombiFlash All-wavelength Collection (PDF: 546K, Jun 2010)
Article Reprints
Networked Purification System Increases Productivity (PDF: 783K, Nov 2006)
Preparative Parallel Protein Purification (PDF: 205K, Mar 2005)
Poster and Paper Reprints
Advanced Topics RediSep Specialty Media (PDF: 1565K, Apr 2007)
Advances In Medium Pressure Liquid Chromatography (PDF: 302K, Mar 2009)
All Wavelength Detection MPLC for Natural Products (PDF: 658K, Jul 2009)
C-18 Chromatography- From Analytical Scale to Preparative Scale (PDF: 355K, Nov 2006)
Diol Columns-Pretend They're Normal Phase (PDF: 657K, Apr 2010)
Facile Enrichment of Tocopherols (PDF: 1107K, Mar 2009)
Facile Isolation of Carotenoid Antioxidants (PDF: 855K, Apr 2010)
Flash Chromatography of Hi pKa Compunds with Specialty RediSep Columns (PDF: 336K, Aug 2004)
Flash Purification of Polar Organic Compounds Using C-18 RediSep (PDF: 138K, May 2005)
Method Development Strategies for Amine Columns (PDF: 505K, Apr 2010)
Minimize Purification Time with High Resolution Flash Chromatography (PDF: 208K, Aug 2004)
Minimizing Protein Purification Time with Parallel Purification (PDF: 824K, Jun 2005)
Optimized Flash Chromatography- From TLC to large scale in 3 steps (PDF: 346K, May 2006)
Purification of Carbohydrates by MPLC (PDF: 886K, Aug 2009)
Sample Loading techniques for Large Scale Flash Chromatography (PDF: 810K, Aug 2010)
Spherical Silica Increases Loading Capacity (PDF: 1428K, Aug 2009)
Presentations
OPopS Optimer One-pot Synthesis (PDF: 193K, Apr 2005)
Tactics Addressing Challenging Flash Chromatography Purifications (PDF: 589K, Apr 2005)
Technical Notes
TN01 Flowcell Care and Cleaning for CombiFlash Companion (PDF: 463K, Jul 2005)
TN02 Networked Purification System Increases Productivity for Medicinal Chemists (PDF: 968K, Apr 2004)
TN03 Companion Direct Communication Issues (PDF: 457K, May 2004)
TN04 Companion Network Communication Issues (PDF: 460K, Jun 2004)
TN05 Companion iPAQ Communication Issues (PDF: 452K, May 2004)
TN06 Controlling the Companion without Wires (PDF: 455K, May 2004)
TN07 Companion Periodic Maintenance (PDF: 174K, Jan 2006)
TN09 Companion Solvent Change between Normal and Reversed Phases (PDF: 128K, Jun 2006)
TN12 Adjustable Solid Load Cartridge Cap (PDF: 255K, Jun 2009)
TN13 Guide to Cartridges and Caps (PDF: 119K, Apr 2009)